Small samples, big data: Microcoring for advanced skin -omics profiling

Summary: Abstract Body: This study introduces microcoring, a minimally invasive biopsy technique, as an innovative tool for -omics research and clinical applications.It offers significant advantages over traditional biopsy methods, which are often associated with discomfort, scarring, and prolonged sequencing times. Combined with STARmap (Spatially Resolved Transcript Amplicon Readout Mapping), a cutting-edge spatial transcriptomics technology, microcoring enables high-resolution gene expression mapping while preserving skin structure organization. Its small sample size supports scalable workflows, and optimized protocols integrating STARmap with H&E staining provide dual-layered gene expression and histology analysis. Microcoring uses a modified hypodermic needle to extract minimally invasive, full-thickness skin samples optimized for -omics workflows. The technique was evaluated for biomolecular preservation, spatial resolution, and feasibility in high-throughput applications like STARmap, which enables spatial mapping of gene expression within intact tissues. Metrics for RNA integrity, protein quantification, and metabolite stability were assessed. Microcoring produced high-quality skin samples with intact biomolecular profiles suitable for -omics analysis. STARmap imaging validated spatially resolved transcriptomic integrity, demonstrating its effectiveness for high-resolution molecular mapping. The minimally invasive approach reduced tissue disruption and variability. Smaller wound size and enhanced patient tolerability support repeated sampling for longitudinal studies and personalized monitoring. Microcoring is a minimally invasive, transformative tool for dermatologic research, enabling scarless sampling while preserving molecular quality. Combining high-resolution -omics capabilities with patient-centered benefits advances understanding of genetic skin conditions, improving diagnostics, therapeutic development, and pathology research. Kenechi Iwelumo^{1, 2}, Jianhuan Qi¹, Ga Ram Ahn¹, Michael McCormack¹, William Austen¹, Jian Shu¹, Dieter Manstein¹ 1. CBRC, Massachusetts General Hospital, Boston, MA, United States. 2. Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ, United States. Translational Studies: Preclinical