Potent complex optimized with tripeptide-32 to increase key proteins and address periorbital skin lifting

Summary: Abstract Body: Research shows that the skin around the eye ages earlier than other sites on the face. Why is it aging faster? This skin is unique and distinct from other areas on the face. Periorbital skin is the most sensitive and delicate skin in our face; it is 40% thinner and as we age, it becomes even thinner due to a natural decrease in protein production. In addition, the skin around the eyes has very few oil glands and therefore it lacks natural moisture. It is also more prone to inflammation and irritation. And finally, the periorbital skin is constantly moving. Blinking (~10,000 times/day) and repetitive facial expressions create fine lines and “crow’s feet”, or lines that appear at the outer corner of the eyes. Therefore, periorbital skin requires specific treatment. In this study, to fight periorbital skin aging by lift this delicate skin, we address key proteins such as elastin, fibrillin, collagen and fibronectin and optimize the treatment result by aligning skin cell functions with the natural circadian rhythm. Ex-vivo skin was treated with different technologies and complexes. After 5 days, treatment with a complex of exclusive Adansonia digitata seed extract+ hexapeptide+algae extract+whey protein (complex 1) did not show significant elastin increase. However, after adding our exclusive tripeptide-32, previously shown to help synchronize skin cell activity with natural circadian rhythm, to this complex, elastin increased by more than 100%, showing once again the importance of synchronization. Results continued to improve: after 10 days, the full complex (complex 1 + tripeptide-32) showed an elastin increase by more than 230% and a fibrillin increase by more than 60%. In addition, in-vitro treatment with the full complex on normal human adult dermal fibroblasts showed an increase in collagen by 113% and fibronectin by 80% after 3 days. We show that the full complex including our exclusive tripeptide-32, is very efficient in increasing key proteins essential to counter periorbital skin sagging. Nadine Pernodet¹, Jacqueline Trivero¹, Sara Byrne¹ 1. Research & Development, Estee Lauder Companies, Melville, NY, United States. Stem Cell Biology, Tissue Regeneration and Wound Healing