Comparison of healing characteristics between dehydrated human amnion/chorion membrane and acellular dermal matrix following mohs micrographic surgical excision for cutaneous malignancies

Summary: This study presents the comparison of healing times following Mohs surgery with the adjunct utilization of dehydrated human amnion/chorion membranes (Epifix) and acellular dermal matrices (ACell-Cytal). This study evaluated a cohort of 77 patients who underwent Mohs surgery. 46 patients received Epifix treatments following surgery and 31 received ACell-Cytal treatments. A retrospective chart review from September 2017 to September 2023 of patient characteristics included: age, race, smoking history, body mass index, wound location, wound size, wound depth, healing time, number of treatments, graft usage, and graft size. Of the 77 patients, 36 were women and 41 were men. The mean age was 74 years. The mean wound size was 2.67cm^2. Patients had a mean of 2.26 treatments in the Epifix cohort and 1.42 treatments in the ACell-Cytal cohort. Both cohorts had a graft usage rate of 74%. The mean healing time from the date of Mohs surgery was 42 days for the Epifix cohort and 31 days for the ACell-Cytal cohort. Statistical analysis using Fisher's exact test comparing the cohorts showed significantly shorter healing times (p < 0.05) and significantly less treatments used (p < 0.001) in the ACell-Cytal cohort, and no significant differences in wound size (p = 0.46). We found patients treated with ACell-Cytal following Mohs surgery had significantly shorter healing times compared to treatment with Epifix. The ACell-Cytal group required significantly less treatments with no significant differences in mean wound size between the two cohorts. Future studies will include larger cohorts controlled for anatomic wound site and depth, comparison of aesthetic outcomes, as well as a cost analysis between the two biological agents as well as secondary healing. Kelly Kaon¹, Thomas Ren¹, Haeun Jung¹, Nicolas Oltean¹, Andre Galenchik-Chan¹, Duc T. Bui^{2, 1}, Sami Khan^{2, 1} 1. Stony Brook University Renaissance School of Medicine, Stony Brook, NY, United States. 2. Division of Plastic and Reconstructive Surgery, Stony Brook University, Stony Brook, NY, United States. Stem Cell Biology, Tissue Regeneration and Wound Healing