Histological and immunohistochemical study of various localization of endometriosis

Summary: Endometriosis is a estrogen–dependent gynecological pathology, defined as the presence of endometrial glands and stroma outside of the uterine cavity, with genital or extragenital localization. It mostly develops in a 10 – 17% of reproductive age and in 25 – 50 % of infertile women. The most common location is within the pelvis. However, extra pelvic endometriosis is a fairly uncommon disorder and difficult to diagnose. It mostly follows abstetrical and gynecologycal surgeries. It presents as a painful, slowly growing mass in or near a surgical scar. In therefore seems that adenomyosis and endometriosis share a common origin in an abnormal ectopic endometrium and miometrium. We studied 13 cases of endometriosis: the anterior abdominal wall after caesarean operation (4), umbilical hernia (1), perineal region (1), intestinal endometriosi (1), uterine adenomyosis (6), age of patients 31 – 59 years. We used hematoxylin – eosin staining, picrofucsin van Gieson and immunohistochemical methods: CD10 marker for endothelial stromal tissue , ER, PR for endometrial gland, Ki 67 cells proliferation markers and CD 68 for macrofages. Obtained dates demonstrate that CD10, ER, PR markers are positive for endometrial tissue. The progesteron receptor antibody showed the strongest positive staining in comparison to the oestrogen receptor. The CD10 antibody had the highest specificity of the stromal cells. Markers CD 68, Ki 67 are negative and can’t be used for the diagnosis. The oestrogen and progesteron receptors, reveal the mechanism of the disease, and determine the most sensitive procedure for detecting an endometrial tissue. Conclusion: CD 10 is the best marker of ectopic endometrial tisssue. Peritoneal endometric lesions and the uterine adenomiosis have a similar immunohistochemical profile. Both endometriotic and endometrial glands are positive for CD 10, ER, PR, but not for Ki 67 or CD 68. Keywords: endometriosis, adenomyosis.